Home
Customized
Rat model generation
Quick KI rats
Rat model generation

Quick KI rats

The Rosa26 and Hprt gene loci are well suited for gene over-expression, reduced development time and cost with ready-to-use targeting vectors.

Contact Us

A Quick Knockin rat line defines a model in which a gene of interest is inserted in the permissive (neutral) locus Rosa26. The transgene can be constitutively or inducibly overexpressed.

Targeting Rosa26 enables the control of the gene copy number and gene expression, and avoids silencing or deregulation of neighboring genes.

Applications

For academic research:

  • Gene function studies
  • Study disease-causing transgene, mutated or not

For bio-pharmaceutical research & development:

  • Compound screening
  • Selectivity screening
  • Drug affinity testing
  • Compound profiling

Strengths of Quick Knockin rat models

  • Stable and predictable expression
  • Almost no position effect variegation
  • Almost no transgene silencing
  • No deregulation of neighbouring gene

Limitations of Quick Knockin rat models

  • Exogenous regulation may result in lower physiological relevancy
  • Limited physiological relevancy when multiple isoforms have been described
  • Problem of stoichiometry if interaction with other partners for signaling
    → Must be solved by an in-depth scientific analysis and a rationale risk assessment prior to model development, all included in our project feasibility assessment
  • Not functional for expression of non-dominant mutations
    → Choose alternative approaches such as, e.g., point mutation Knockin

Validation

Related resources and publications
Posters
(
0
)
Scientific Articles
(
0
)
Commentaires
(
0
)
Case studies
(
0
)
No results
No items found.
Efficient generation of human immune system rats using human CD34+ cells

Stem Cell Reports

Aug 2024
Early lysosome defects precede neurodegeneration with amyloid-β and tau aggregation in NHE6-null rat brain

Brain

Dec 2021
The Lrat-/- Rat: CRISPR/Cas9 Construction and Phenotyping of a New Animal Model for Retinitis Pigmentosa

Int J Mol Sci

Jul 2021
Long Acellular Nerve Allografts Cap Transected Nerve to Arrest Axon Regeneration and Alter Upstream Gene Expression in a Rat Neuroma Model

Plast Reconstr Surg

May 2021
Comparison of Myelin-Associated Glycoprotein With Vincristine for Facial Nerve Inhibition After Bilateral Axotomy in a Transgenic Thy1-Gfp Rat Model

JAMA Facial Plast Surg

May 2019
Assessment of fear and anxiety associated behaviors, physiology and neural circuits in rats with reduced serotonin transporter (SERT) levels

Transl Psychiatry

Jan 2019
Constitutive serotonin transporter reduction resembles maternal separation with regard to stress-related gene expression

ACS Chem Neurosci

Nov 2018
5-HT3 receptor signaling in serotonin transporter knockout rats: a female sex specific animal model of visceral hypersensitivity.

Am J Physiol Gastrointest Liver Physiol

Oct 2018
Generation of immunodeficient rats with Rag1 and Il2rg gene deletions and human tissue grafting models.

Transplantation

Apr 2018
Acute and subchronic antinociceptive effects of nociceptin/orphanin FQ receptor agonists infused by intrathecal route in rats.

Eur J Pharmacol

Jan 2015
Serotonin Reuptake Transporter Deficiency Modulates the Acute Thermoregulatory and Locomotor Activity Response to 3,4-({+/-})-Methylenedioxymethamphetamine, and Attenuates Depletions in Serotonin Levels in SERT-KO Rats

Toxicol Sci

Mar 2014
Limited regeneration in long acellular nerve allografts is associated with increased Schwann cell senescence.

Exp Neurol

Sep 2013
Nitric oxide regulates cardiac intracellular Na+ and Ca2+ by modulating Na/K ATPase via PKCε and phospholemman-dependent mechanism.

J Mol Cell Cardiol

Aug 2013
Visceral hypersensitivity in female but not in male serotonin transporter knockout rats.

Neurogastroenterol Motil

Jun 2013
Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases.

FASEB J

Feb 2013
Mesenchymal stem cell therapy prevents IFTA in a rat kidney allograft model

Stem Cells Dev

Nov 2012
Geldanamycin accelerated peripheral nerve regeneration in comparison to FK-506 in vivo.

Neuroscience

Oct 2012
A transgenic rat expressing green fluorescent protein (GFP) in peripheral nerves provides a new hindlimb model for the study of nerve injury and regeneration

J Neurosci Methods

Feb 2012
Effects of Human Alpha-Synuclein A53T-A30P Mutations on SVZ and Local Olfactory Bulb Cell Proliferation in a Transgenic Rat Model of Parkinson Disease

Parkinsons Dis

Jun 2011
Lack of the serotonin transporter (SERT) reduces the ability of 5-hydroxytryptamine to lower blood pressure.

Naunyn Schmiedebergs Arch Pharmacol

May 2011
Comparison of the function of the serotonin transporter in the vasculature of male and female rats

Clin Exp Pharmacol Physiol

May 2011
Pharmacological blockade or genetic knockout of the NOP receptor potentiates the rewarding effect of morphine in rats.

Drug Alcohol Depend

Apr 2011
Nociceptin/orphanin FQ receptor knockout rats: in vitro and in vivo studies.

Neuropharmacology

Mar 2011
Serotonin transporter interacts with the PDGFβ receptor in PDGF-BB-induced signaling and mitogenesis in pulmonary artery smooth muscle cells

Am J Physiol Lung Cell Mol Physiol

Mar 2011
A New Model for Facial Nerve Research: the novel transgenic Thy1-GFP rat

Arch Facial Plast Surg

Sep 2010
Identification of genetic modifiers of behavorial phenotypes in serotonin transporter knockout rats

BMC Genetics

May 2010
Body distribution of infused serotonin in rats.

Clin Exp Pharmacol Physiol

May 2009
No results
No results
No results
No results
No results

Discover related products to

Quick KI rats

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
Customized rat

Point mutation KI rats

Use a point mutation rat Knockin to circumvent complex phenotypes arising from complete Knockouts (e.g., signaling pathway problems, cross-reactivity).

Customized rat

Constitutive KO rats

Use a constitutive Knockout rat to permanently inactivate your target gene; in the whole animal, in every cell of the organism, effective at all stages of development, from the one-cell embryo stage through adulthood.

Customized rat

Tissue-specific KO rats

Use  tissue- or cell-specific conditional Knockout rat models to bypass embryonic lethality,  compensatory mechanisms, complex phenotypes, etc.

Customized rat

Time-dependent KO rats

Use an inducible conditional Knockout rat to age-dependently inactivate your gene, and to enable studies at defined development stages or on age-related diseases.

Discover our off-the-shelf models.

Learn more

Quick Knockin rat models (Neutral locus targeting: Rosa26)

Get in touch about

Let us know how we can help

In order to provide you the content requested, we need to store and process your personal data. If you consent to us storing your personal data for this purpose, please tick the checkbox below.

From time to time, we would like to contact you about our products and services, as well as other content that may be of interest to you. If you consent to us contacting you for this purpose, please tick below.

You can unsubscribe from these communications at any time. For more information on how to unsubscribe, our privacy practices, and how we are committed to protecting and respecting your privacy, please review our Privacy Policy.

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.