Related resources and publications
Targeting the human CD47/SIRPα axis
Humanized ICP mouse and cell lines
Front Immunol
PLoS One
Immunology
The EL-4 cell line was established from a C57BL/6 murine lymphoma. These cells form tumors in syngeneic models, upon subcutaneous and systemic (i.v.) injection, in immunocompetent mice.
We generated an EL4-hCD19-LZ clonal cell line expressing high levels of human CD19 (NCBI GeneID: 930), and an optimized dual fusion reporter luciferase-ZsGreen (LZ). EL4-hCD19-LZ cells form tumors in vivo when injected subcutaneously. Luciferase-ZsGreen (LZ) control cells are available.
Expression analysis by flow cytometry. Human CD19 expression was assessed in EL4 parental cell line (left), human control cells – PBMC (center), and EL4-hCD19-LZ cell line (right).
Left figure. Humanized CD3 C57/Bl6 mice were injected (s.c.) with 1x106 EL4-hCD19-LZ cells. Tumor growth was measured every 2 to 4 days. Tumor size (mm3) = (width² x length)/2.
Right figure. Medium (200 mm3) and large (800 mm3) tumors were collected and tumor composition was determined by flow cytometry.
Targeting the human CD47/SIRPα axis
Humanized ICP mouse and cell lines
Front Immunol
PLoS One
Immunology
Let us know how we can help
Physiological relevance
Comprehensive and reliable validation data
Over 600 scientific articles based on our models
In-depth expertise in preclinical science, physiology, and model development
Unique R&D platform located in France
Tailor-made approach to your needs
Strong emphasis on customer satisfaction
Long-standing partnerships with 17 of the top 20 pharma companies
Co-development of innovative models for next-generation drugs
Guaranteed FTO/freedom to operate