Design of the hPD-1/hPD-L1 mouse
The double-humanized PD‑1 and PD-L1 (hPD-1/hPD-L1) mouse model was generated by intercrossing hPD-1 and hPD-L1 mice.
The PD-1 humanized model, developed by Knockin at the mouse Pd-1 locus, expresses a chimeric PD-1: human extracellular and mouse transmembrane and intracellular domains.
The hPD-L1 humanized model, developed by Knockin at the mouse Pd-L1 locus, expresses a chimeric PD-L1 (human extracellular domains and murine transmembrane and intracellular domains).
Both hPD-1 and hPD-L1 expressions are regulated by endogenous mouse promoters.
hPD-1/hPD-L1 features
- PD-1 and PD-L1 extracellular domains are entirely humanized
- hPD-1 and hPD-L1 expression driven by the respective endogenous mouse promoter
- Preservation of the target-ligand interaction
- Fully functional mouse immune system
- Lack of expression of the murine target gene, thus avoiding cross-reactivity