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genO-hPD-1/hGITR/hGITRL
Humanized mice (target-specific)

Preclinical triple-humanized genO-hPD-1/hGITR/hGITRL mouse model

The genO‑hPD‑1/hGITR/hGITRL mouse model enables in vivo efficacy assessment and profiling of antibodies targeting human immune checkpoint PD-1, GITR and/or GITRL in fully immunocompetent mice.

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Design of the genO‑hPD‑1/hGITR/hGITRL mouse

The triple-humanized PD‑1, GITR and GITRL mouse model (genO-hPD-1/hGITR/hGITRL) was generated by intercrossing genO-hPD-1, genO-hGITR and genO-hGITRL mice.‍

genO-hPD-1 was generated by inserting, within the mouse Pd-1 locus, a chimeric PD-1 with the human extracellular and murine transmembrane and intracellular domains.

genO-hGITR was developed by inserting, within the mouse Gitr locus, a chimeric GITR with the human extracellular and the murine intracellular domain.

genO-hGITRL was generated by inserting, within the mouse Gitrl locus, a chimeric GITRL with the human extracellular and the murine intracellular domain.

hPD-1, hGITR and hGITRL expressions are regulated by endogenous mouse promoters.

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genO-hPD-1/hGITR/hGITRL features

  • PD-1, GITR and GITRL extracellular domains are entirely humanized
  • hPD-1, hGITR and hGITRL expression driven by the respective endogenous mouse promoter
  • Fully functional mouse immune system
  • Lack of expression of the murine target genes, thus avoiding cross-reactivity

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