Design of the hPD-1/hLAG3 mouse
The double-humanized PD‑1 and LAG3 (hPD-1/hLAG3) mouse model was generated by intercrossing hPD-1 and hLAG3 mice.
The PD-1 humanized model, developed by Knockin at the mouse Pd‑1 locus, expresses a chimeric PD‑1: human extracellular and mouse transmembrane and intracellular domains.
The LAG3 humanized model, developed by Knockin at the mouse Lag3 locus, expresses the full-length chimeric LAG3 isoform (human extracellular domains, and mouse transmembrane and intracellular domains).
Both hPD-1 and hLAG3 expressions are regulated by endogenous mouse promoters.
hPD-1/hLAG3 features
- PD-1 and LAG3 extracellular domains are entirely humanized
- hPD-1 and hLAG3 expression driven by the respective endogenous mouse promoter
- Fully functional mouse immune system
- Lack of expression of the murine target gene, thus avoiding cross-reactivity